University of Texas System

The Texas A&m University System

Aaron L Miller

Richard Pyles

Robert E Garfield

Alexandru T Balaban

William A Seitz

Marc A C Ilies

Edward Bradbridge Thompson

Betty H Johnson

Melissa J Wentz

Certain embodiments of the invention include methods and compositions for evaluating flaviviruses, such as Zika virus, for the purpose of identifying, typing, and/or categorizing/speciation of virus in samples using nucleic acid sequencing.

Molecular typing system for flavivirus diagnostics

Certain embodiments of the invention include, but are not limited to PCR primer pairs, sequencing primers, and/or associated thermocycling protocols targeting a region identified by the inventors within the 5' untranslated region of enteroviruses for the purpose of identifying, subtyping, and/or classifying of virus in samples using nucleic acid sequencing. The sequencing procedures can use nucleic acid templates, such as cDNA or PCR amplicons, as a template for sequencing in medium to high throughput format that is cost effective and easily deployed to other clinical microbiology laboratories.

Methods and compositions for identifying enterovirus

Pyridinium cationic lipids useful as non-viral gene delivery agents are disclosed. The agents are prepared by reaction of pyrylium salts with primary amines. Also disclosed are methods of trasfectind cells using the pyridinium cationic lipids as gene transfer agents.

Pyridinium cationic lipids as gene transfer agents

Growing community of inventors

Galveston, TX, United States of America

Aaron L Miller