Harvard College

Aaron L Glieberman

Kevin Kit Parker

Benjamin D Pope

Nina R Sinatra

Alanna R Gannon

John F Zimmerman

Kevin L Shores

John P Ferrier

Some embodiments provide a microfluidic cartridge for automatically hydrodynamically loading objects (e.g., cell clusters) into traps in parallel trapping channels with one object per trap, methods of making such cartridges and methods of use of such cartridges.

Microfluidic trapping chip and uses thereof for culture and assay of cell clusters and objects

Microfluidic systems including a housing configured to receive a cartridge, the cartridge including a plurality of wells for receiving one or more biological cells are described herein. The microfluidic systems include a structure with channels for introducing a medium into the housing and through channels the cartridge. Microfluidic systems including a housing configured to receive a cartridge, the housing including channels for loading one or more biological cells within wells of the cartridge are described herein. Three-dimensional scaffolds, devices, and systems comprising such scaffolds that mimic the in vivo structure, physical properties, and protein composition of extracellular matrix surrounding pancreatic islet cells and adipocytes, and the use of such compositions, devices and systems for, e.g., in vitro drug screening and/or toxicity assays, disease modeling, and therapeutic applications are also described herein.

Cartridge-based system for long term culture of cell clusters

Growing community of inventors

Cambridge, MA, United States of America

Aaron L Glieberman