Handylab, Inc.

Bio-rad Laboratories, Inc.

Akadeum Life Sciences, Inc.

Accuri Cytometers, Inc.

Denovo Sciences, Inc.

Blaze Medical Devices, LLC

Celsee Diagnostics, Inc.

Aaron Kehrer

Gene Parunak

Kalyan Handique

Betty Wu

Sundaresh N Brahmasandra

Karthik Ganesan

Priyadarshini Gogoi

Kyle Gleason

Christopher Siemer

Saedeh Sepehri Javdani

Nikhil Phadke

John S Althaus

Cecelia Haley

Ted Springer

Jon Meines

Brandon H McNaughton

John G Younger

Leo Ostruszka

Jonathan Roussey

Jeff Williams

Nathaniel C Bair

Kenneth Alfano

Michael Tarasev

Terrance Boyd

Steven Meines

Collin Anderson Rich

Jack T Ball

Kaylan Handique

A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.

System for imaging captured cells

A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.

Processing particle-containing samples

A method and system for buoyant separation of a target constituent of a sample, the method comprising: at a process chamber, combining a volume of substrates having a first density with the sample, thereby producing a population of target-bound complexes comprising the target constituent bound to at least a portion of the volume of substrates; within the process chamber, physically separating the population of target-bound complexes from the sample based upon interaction between the volume of substrates and an applied force; aggregating the population of target-bound complexes at a collection region of the process chamber; extracting the population of target-bound complexes from the process chamber; and processing the target constituent from the population of target-bound complexes for further analysis.

Method and system for buoyant separation

Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.

Method for processing polynucleotide-containing samples

A bead mill and an associated bead-mill-based machine for testing mechanical fragility of red blood cells, employing a cartridge configured to contain a sample while cells get stressed via bead oscillation and, in the case of the fragility testing machine, also while lysis levels get detected, for presentation of fragility information.

Method of using bead milling in erythrocyte fragility testing

System for processing polynucleotide-containing samples

Bead mill and method of use

Growing community of inventors

Ypsilanti, MI, United States of America

Aaron Kehrer