Leica Biosystems Imaging, Inc.

Other

Aaron Alan Stearrett

Peyman Najmabadi

Nicholas Newberg

Prentash Djelosevic

Dirk G Soenksen

Allen Olson

Greg J Crandall

Yunlu Zou

Gregory J Crandall

Leng-Chun Chen

Timothy Dailey

Christopher Adam Lee

Cynthia Perz

A stall detection system is provided that determines if a glass slide in motion during an automated process is at risk for being damaged and stops motion in the event of an unacceptable risk of damage. The system includes one or more motors configured to move a glass slide (directly or indirectly). The motors are configured to generate a load resistance value. The system includes one or more processors that monitor the load resistance value of a motor during motion and compares the load resistance value to a predetermined threshold resistance value to determine the risk of a glass slide being damaged. The predetermined threshold resistance value may correspond to a risk of slide breakage in response to a force applied to any surface of the slide, or a risk of losing a controlling grip on a slide rack, or a risk of a motor skipping a motor step.

Stall detection for autoloader axes

A slide rack inventory and reinsertion system for use with a digital slide scanning apparatus is provided that determines, prior to scanning of glass slides in a slide rack, a status of each slot in the slide rack as properly occupied, improperly occupied, or empty. The system also determines whether a slide that has been removed from the slide rack for processing has been properly reinserted into the slide rack.

Slide inventory and reinsertion system

A stuck slide determination system is provided that determines if a slide (e.g., glass slide), positioned on a scanning stage at the start of the scanning process, gets stuck and does not move along with the scanning stage after the scanning process commences. In an embodiment, the system includes a sensor having a transmitter and receiver that are relatively positioned to detect the presence of the stage or a slide or the absence of the stage or a slide. When the scanning stage begins to move at the start of the scanning process, a processor monitors a signal from the sensor and determines if the slide is improperly positioned after the scanning process has commenced. If the slide is improperly positioned, the processor stops movement of the scanning stage in order to protect the slide from damage.

Stuck slide determination system

Systems and methods for managing a plurality of scanning devices in a high-throughput laboratory environment. Each of the scanning devices is configured for a remote boot operation from an administrative server that is communicatively coupled with the plurality of scanning devices via a local network. The remote boot replaces the complete operational firmware of a scanning device. The scanning devices are each configured to periodically provide operational information to the administrative server for centralized storage. The centralized storage of operational information for each of the plurality of scanning devices, coupled with the ability of the administrative server to initiate a reboot of any scanning device and thereby update the complete operational firmware of the scanning device, allows for centralized administration of multiple scanning devices that facilitates configuration, support, image data storage, and/or communication with outside servers.

Managing plural scanning devices in a high-throughput laboratory environment

Two-pass capture of a macro image. In an embodiment, a scanning apparatus comprises a stage, a high-resolution camera, and a lens that provides a field of view, substantially equal in width to a slide width, to the high-resolution camera. The apparatus also comprises a first illumination system for transmission-mode illumination, and a second illumination system for reflection-mode illumination. Processor(s) move the stage in a first direction to capture a first macro image of a specimen during a single pass while the field of view is illuminated by the first illumination system, and move the stage in a second direction to capture a second macro image of the specimen during a single pass while the field of view is illuminated by the second illumination system. The processor(s) identify artifacts in the second macro image, and, based on those artifacts, correct the first macro image to generate a modified first macro image.

Two pass macro image

A system for creating a contiguous digital image of a fluorescence microscope sample. In an embodiment, the system comprises a both a macro camera and a time delay integration (TDI) line scan camera. The system may also comprise a motorized stage, an illumination module, a light source, an excitation filter, an objective lens, an emission filter, and at least one processor configured to assemble a plurality of digital images of portions of the fluorescence microscope sample, generated by the TDI line scan camera, into a contiguous digital image of at least a portion of the fluorescence microscope sample.

Whole slide fluorescence scanner

A whole slide fluorescence digital pathology system is provided that uses a monochrome TDI line scan camera, which is particularly useful in fluorescence scanning where the signal is typically much weaker than in brightfield microscopy. The system uses oblique brightfield illumination for fast and accurate tissue finding and employs a unique double sweep focus scoring and objective lens height averaging technique to identify focus points and create a focus map that can be followed during subsequent scanning to provide autofocusing capability. The system also scans and analyzes image data to determine the optimal line rate for the TDI line scan camera to use during subsequent scanning of the digital slide image and it also creates a light profile to compensate for loss of illumination light due to roll off.

Growing community of inventors

Vista, CA, United States of America

Aaron Alan Stearrett