Inscripta, Inc.

Aamir Mir

Andrew Garst

Kyle Seamon

Benjamin Mijts

Juhan Kim

Stephen Federowicz

Phillip Belgrader

Jorge Bernate

Eric D Smith

Bruce Chabansky

Christian Siltanen

David Stumbo

Nathan Bade

Burak Dura

Xi-Jun Chen

Janine Mok

Brian Chaikind

Emily Feldman

Erik Zimmerman

The present disclosure provides compositions of matter, methods and instruments for nucleic acid-guided nickase/reverse transcriptase fusion enzyme editing of nucleic acids in live mammalian cells.

Dual strand nucleic acid-guided nickase editing

This invention relates to compositions of matter, methods, modules and automated, end-to-end closed instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells. The disclosed compositions and method entail making 'reagent bundles' comprising many (hundreds of thousands to millions) clonal copies of an editing cassette and delivering or co-localizing the reagent bundles with live mammalian cells such that the editing cassettes edit the cells and the edited cells continue to grow.

Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells using microcarriers

The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

Cascade/dCas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells

The present disclosure provides engineered RNA-guided enzymes for editing live cells.

Engineered enzymes

The present disclosure provides new RNA-guided nucleases for making rational, direct edits to nucleic acids in live cells; specifically, the present disclosure provides Type V MAD nucleases (e.g., RNA-guided nucleases or RGNs) with altered PAM preferences and/or altered activity at different temperatures or fidelity, and/or varied nuclease activities; all changes that may increase the versatility of a nucleic acid-guided nuclease for certain editing tasks.

Mad nucleases

The present disclosure provides new RNA-guided nuclease systems and engineered nickases for making rational, direct edits to nucleic acids in live cells.

The present disclosure provides new RNA-guided nucleases for making rational, direct edits to nucleic acids in live cells.

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Boulder, CO, United States of America

Aamir Mir