Abbott Laboratories Corporation

Icahn School of Medicine at Mount Sinai

A Scott Muerhoff

Suresh M Desai

Christopher C Marohnic

George J Dawson

David J Hawksworth

Robert N Ziemann

Thomas P Leary

Bryan C Tieman

Robin A Gutierrez

Kathy Otis

April Ahlberg

Dinesh O Shah

John C Prostko

Isa K Mushahwar

Larry G Birkenmeyer

James L Stewart

Lily Jiang

Ronald L Marshall

John N Simons

Andrea Branch

Cynthia Jou

M Felicia Bogdan

Francis Eng

Robin A Guetierrez

M Felisha Bogdan

Furesh M Desai

Bryan Tieman

George A Dawson

The present disclosure provides detection methods employing HCV core lipid binding domain and DNA binding domain monoclonal antibodies. In certain embodiments, the lipid binding domain monoclonal antibody recognizes an epitope in amino acids 141 to 161 of HCV core protein.

Detection methods employing HCV core lipid and DNA binding domain monoclonal antibodies

The present disclosure provides detection methods employing HCV core lipid binding domain and DNA binding domain monoclonal antibodies or antibody fragments. In certain embodiments, the lipid binding domain monoclonal antibody or antibody fragment recognizes an epitope in amino acids 141 to 161 of HCV core protein and the DNA binding domain antibody or antibody fragment recognizes an epitope in amino acids 95-123 (e.g., in amino acids 99-117) of HCV core protein.

The present invention relates to monoclonal antibodies for the detection of HCV antigen. More specifically, the invention describes antibodies against HCV core antigen lipid binding domain and immunoassay methods, kits and compositions for use in detecting HCV infection.

HCV core lipid binding domain monoclonal antibodies

The present invention generally relates to combination immunoassays, reagents and kits for simultaneous detection of HCV antigens and anti-HCV antibodies in a test sample.

HCV antigen-antibody combination assay and methods and compositions for use therein

The present disclosure relates to polypeptides, including fusions thereof, nucleic acids, vectors, host cells, immunodiagnostic reagents, kits, and immunoassays for use detecting the presence of HCV antibodies. More specifically, the present invention describes specific NS3 antigens that can be used for the detection of anti-HCV antibodies.

HCV NS3 recombinant antigens and mutants thereof for improved antibody detection

A polypeptide comprising the contiguous amino acids 1-198 of SEQ ID NO: 2; a polypeptide, which comprises a contiguous amino acid sequence that is at least about 95% identical to the contiguous amino acids 1-198 of SEQ ID NO: 2, an epitope that is immunoreactive with an antibody that specifically binds to the core protein of hepatitis C virus (HCV), and an epitope that is immunoreactive with an antibody that specifically binds to the NS4 region of HCV; a nucleic acid encoding such a polypeptide; a host cell comprising such a nucleic acid; an immunodiagnostic reagent comprising such a polypeptide; a kit comprising such an immunodiagnostic reagent; and a method of determining the presence, amount, or concentration of anti-HCV antibodies in a test sample.

Materials and methods for assay of anti-hepatitis C virus (HCV) antibodies

The subject invention relates to methods for the simultaneous detection of Hepatitis C Virus (HCV) antigens as well as antibodies produced in response to HCV antigens. Furthermore, the subject invention allows one to detect antigens in the early, acute stage of infection, even prior to the development of antibodies, thereby allowing for early detection of infected blood and blood products, thus improving the safety of the blood supply.

Methods for the simultaneous detection of HCV antigens and HCV antibodies

The subject invention relates to improvements in nucleic acid isolation, and more particularly, relates to modifications to the subtractive hybridization method and to reagents such as oligonucleotides that are useful when performing the method.

Method of performing subtractive hybridization using RDA

Nucleic oligomer probes useful for detection of HGBV in test samples. Also provided are assays which utilize these probes and test kits which contain these oligomer probes.

Nucleic acid detection of hepatitis GB virus

Growing community of inventors

Kenosha, WI, United States of America

A Scott Muerhoff