Miles Laboratories, Inc.

Miles Inc.

A Christopher Skjold

Lonnie R Stover

Gerhard Wolfrum

Herbert Hugl

Paul F Corey

James Travis

Eugen Schnabel

James H Pendergrass

Robert W Trimmer

Agent for the detection of esterolytic and/or proteolytic enzymes, containing (a) an amino acid ester or peptide ester of a phenol, as the chromogenic enzyme substrate, and (b) an alcohol as the substance which accelerates the enzymatic cleavage of the amino acid ester bond or peptide ester bond of component (a), characterized in that it contains a salt as the accelerating substance.

Analytical process and agents for the detection of esterolytic and/or

A composition for determining the presence of leukocytes, esterase or protease in a test sample. The composition comprises an ester having the structure ##STR1## in which: A is an N-blocked-amino acid residue or an N-blocked-peptide residue, X is O, S or NR', R is aryl, or lower alkyl, R* is H, or lower alkyl, and R' is H, lower alkyl or aryl; and a suitable buffer substance.

Composition and test device for determining the presence of leukocytes,

A composition and test device for determining the presence of an analyte selected from leukocytes, esterase and protease in a test sample. The composition comprises an ester capable of producing a detectable response upon interaction with said analyte, and a diazonium salt having the structure ##STR1## in which A.sup.- is an anion, and R same or differ, is H, lower alkyl, aryl, or both of R together form a fused ring system, and in which R' is H, OH or lower alkyl.

Composition and test device for determining the presence of leukocytes

A composition, test device and method for determining the presence of leukocytes, esterase and/or esterase activity in a test sample is disclosed. The composition comprises an ester which, when cleaved by esterolytic activity, produces a detectable response such as color formation. In addition the composition comprises the compound 3-quinuclidinol. The device is prepared by incorporating a carrier matrix, such as paper, with the composition. The method comprises contacting a test sample with the device and observing a detectable response.

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Elkhart, IN, United States of America

A Christopher Skjold